Our Leaders -
Technology & Design

Research interests

• Regenerative medicine and Cell Therapy
• Synthetic biology
• Molecular biology

Professional activity

2015.09 - current

Member of Special Committee on Regenerative Medicine, Tokai University

2015.12 - 2017.12

Member of the Ethics Review Committee, Japan Biological Informatics Consortium (JBIC)

2017.04 - current

Member of the Committee on Nanotechnology and Materials Science, Ministry of Education, Culture, Sports, Science and Technology, Japan

2018.06 - current

Officer, Kobe Port Island Drug Discovery Forum

2018.06 - current

Member, Operations Committee, Kobe Biomedical Innovation Cluster

2021.04 - current

Member, ISCT APAC Committee

Graduate school lectures

University of Tokyo, Hokkaido University, Tokyo University of Agriculture, Ritsumeikan University

Major publications

1. Saito H, Kato M, Hirai K, Kiyama M, Ohyama K, Hanzawa H, Nakane A, Sekiya S, Yoshida K, Kishino A, Tsuchida A, Kimura T, Takahashi J, and Takeda S: Analysis of extracellular vesicles as a potential index for monitoring differentiation of neural lineage cells from induced pluripotent stem cells, J Biosci Bioeng, 132(4), 381-389, 2021.
2. A.Nishimura, R,Nakajima, R.Takagi, G.Zhou, D.Suzuki, M.Kiyama, T.Nozaki, T.Owaki, T.Takahara, S.Nagai, T.Nakamura, M.Sugaya, K.Terada, Y.Igarashi, H.Hanzawa, T.Okano, T.Shimizu, M.Yamato and S.Takeda: Fabrication of tissue-engineered cell sheets by automated cell culture equipment, J Tissue Eng Regen Med., 13(12): 2246-2255, Dec 2019.
3. M.Okanojo, K.O.Okeyo, H.Hanzawa, O.Kurosawa, H.Oana, S.Takeda and M.Washizu: Nuclear transplantation between allogeneic cells through topological reconnection of plasma membrane in a microfluidic system, Biomicrofluidics, 13(3):034115, 10 June 2019.
4. E.Matsumoto, N.Koide, H.Hanzawa, M.Kiyama, M.Ohta, J.Kuwabara, S.Takeda and M.Takahashi: Fabricating retinal pigment epithelial cell sheets derived from human induced pluripotent stem cells in an automated closed culture system for regenerative medicine, PLoS One., 14(3):e0212369, 13 Mar 2019.
5. T.Kikuchi, M.Kino-Oka, M.Wada, T.Kobayashi, M.Kato, S.Takeda, H.Kubo, T.Ogawa, H.Sunayama, K.Tanimoto, M.Mizutani, T.Shimizu and T.Okano: A novel, flexible and automated manufacturing facility for cell-based health care products: Tissue Factory. Regen Ther., 9:89-99, 29 Sep 2018.
6. R.Nakajima and S.Takeda: Efficient fabrication of epidermal cell sheets using γ-secretase inhibitor, J Dermatol Sci., 76(3):246-54, Dec 2014.
7. R.Nakajima and S.Takeda: Fabrication of corneal epithelial cell sheets maintaining colony-forming cells without feeder cells by oxygen-controlled method, Exp Eye Res., 118:53-60, Jan 2014.
8. Y.Oie, T.Nozaki, H.Takayanagi, S.Hara, R.Hayashi, S.Takeda, K.Mori, N.Moriya, T.Soma, M.Tsujikawa, K.Saito and K.Nishida, Development of a cell sheet transportation technique for regenerative medicine. Tissue Eng Part C Methods. 20(5):373-82, May 2014.
9. R.Nakajima and S.Takeda: The efficient fabrication of corneal epithelial cell sheets by controlling oxygen concentration, Exp Eye Res., 116:434-8, Nov 2013.
10. R.Nakajima, T.Kobayashi, T.Kikuchi, Y.Kitano, H.Watanabe, M.Mizutani, T.Nozaki, N.Senda, Saitoh R.Takagi, M.Yamato T.Okano, S.Takeda: Fabrication of transplantable corneal epithelial and oral mucosal epithelial cell sheets using a novel temperature-responsive closed culture device. J Tissue Eng Regen Med., 9(5):637-40, May 2015.
11. R.Nakajima, T.Kobayashi, N.Moriya, M.Mizutani, K.Kan, T.Nozaki, K.Saitoh, M.Yamato, T.Okano and S.Takeda: A novel closed cell culture device for fabrication of corneal epithelial cell sheets, J Tissue Eng Regen Med., 9(11):1259-67, Nov. 2015.
12. Y.Ishige, S.Takeda and M.Kamahori: Direct detection of enzyme-catalyzed products by FET sensor with ferrocene-modified electrode, Biosens Bioelectron., 26(4):1366-72. doi: 10.1016/j.bios.2010.07.053. E, published 21 Jul 2010.
13. H.Suzuki et al., The transcriptional network that controls growth arrest and differentiation in a human myeloid leukemia cell line, FANTOM Consortium, Nat Genet., 41(5):553-62, May 2009.
14. T.Urano, T.Usui, S.Takeda et al.: TRIM44 interacts with and stabilizes terf, a TRIM ubiquitin E3 ligase, Biochem Biophys Res Commun.;383(2):263-8, 29 May 2009.
15. T.Miyasaka, M.Morita, K.Ito, T.Suzuki, H.Fukuda, S.Takeda, et al.: Interaction of antiproliferative protein Tob with the CCR4-NOT deadenylase complex, Cancer Sci., 99(4):755-61, April 2008.
16. N.Sugaya, K.Ikeda, T.Tashiro, S.Takeda et al.: An integrative in silico approach for discovering candidates for drug-targetable protein-protein interactions in interactome data, BMC Pharmacol., 7:10, 20 August 2007.
17. Y.Sano, T.Nakaya, S.Pedrini, S.Takeda, et al., Physiological mouse brain Abeta levels are not related to the phosphorylation state of threonine-668 of Alzheimer's APP, PLoS One., 1:e51, 20 Dec 2006.
18. T.Suzuki, K.Ando, K.Iijima, S.Oguchi and S.Takeda: Phosphorylation of Amyloid Precursor Protein (APP) Family Proteins, Methods Mol Med.,32:271-82. doi: 10.1385/1-59259-195-7:271, 2000.
19. K.Iijima, K.Ando, S.Takeda et al.: Neuron-specific phosphorylation of Alzheimer's beta-amyloid precursor protein by cyclin-dependent kinase, J Neurochem., 75(3):1085-91, Sept. 2000.
20. K.Ando, M.Oishi, S.Takeda et al.: Role of phosphorylation of Alzheimer's amyloid precursor protein during neuronal differentiation, J Neurosci., 19(11):4421-7, 1 June 1999.
21. S.Tomita, T.Ozaki, H.Taru, S.Oguchi, S.Takeda et al.: Interaction of a neuron-specific protein containing PDZ domains with Alzheimer's amyloid precursor protein, J Biol Chem., 274(4):2243-54, 22 Jan 1999.
22. J.Desdouits-Magnen, F.Desdouits, S.Takeda et al.: Regulation of secretion of Alzheimer amyloid precursor protein by the mitogen-activated protein kinase cascade, J Neurochem., 70(2):524-30, Feb 1998.
23. T.Okamoto, S.Takeda et al.: Intrinsic signaling function of APP as a novel target of three V642 mutations linked to familial Alzheimer's disease, EMBO J., 15(15):3769-77, 1 Aug 1996.
24. T.Yamatsuji, T.Matsui, T.Okamoto, K.Komatsuzaki, S.Takeda et al.: G protein-mediated neuronal DNA fragmentation induced by familial Alzheimer's disease-associated mutants of APP, Science, 272(5266):1349-52, 31 May 1996.
25. T.Yamatsuji, T.Okamoto, S.Takeda et al.: Expression of V642 APP mutant causes cellular apoptosis as Alzheimer trait-linked phenotype, EMBO J., 15(3):498-509, 1 Feb 1996.
26. Y.Murayama, S.Takeda et al.: Cell surface receptor function of amyloid precursor protein that activates Ser/Thr kinases, Gerontology, 42 Suppl 1:2-11, 1996.
27. T.Okamoto, S.Takeda et al.: Ligand-dependent G protein coupling function of amyloid transmembrane precursor, J Biol Chem., 270(9):4205-8, 2 March 1995.
28. S.Takeda et al.: Purification of low-molecular-weight GTP-binding proteins structurally related to MTG33, a mitochondrial GTP-binding protein of Ehrlich ascites tumor cells, J Biochem., 118(4):791-5, Oct 1995.
29. S.Takeda et al.: Purification of a GTP-binding protein localized in mitochondria, J Biochem., 114(5):684-90, Nov 1993.
30. H.Hirai, S.Takeda et al.: Inhibition of SV40 DNA replication in vitro by chlorpromazine, Biol Pharm Bull., 16(6):565-7, June 1993.
31. S.Takeda et al.: Nuclear GTP-binding proteins of Swiss 3T3 cells, FEBS Lett., 244(2):469-72, 27 Feb 1989.

Major awards

• 2018 60th Best 10 New Products Award, among others

Invited lectures (outside Japan)

2019

Trends for cell culture technology and automation in regenerative medicine, Chang Gung Memorial Hospital Conference 2019

2018

Hitachi R&D for Promotion of Regenerative Medicine, 2018 Healthcare Expo Taiwan

2010

Hitachi Group's Approach to Regenerative Medicine, 2010 International Society for Cellular Therapy 1st Asia-Pacific Regional Meeting 2010